Evaluation of the Abbott RealTime HCV Genotype II kit

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چکیده

The Abbott RealTime HCV Genotype II kit is designed for use in the transcription and amplification of the 5’UTR or NS5B regions of the hepatitis C virus (HCV) genome. RealTime-PCR amplification is conducted in three separate reaction mixtures with different fluorophores where genotypespecific probes are used to detect HCV genotypes 1, 1a, 1b, 2, 3, 4, 5 and 6 such that: • Reaction A detects all HCV isolates, subtype 1a and genotype 3 • Reaction B detects genotype 2, subtype 1b and genotype 1 • Reaction C detects genotype 4, 5 and 6.

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تاریخ انتشار 2011